Analysis of Gly aminoacyl synthetase.

Analysis performed on 2024-09-30 10:30:08

Number of Proteins Analyzed

Initial Filtered Out Final Blast
Bacteria 64500 753 63747 11946
Archaea 3183 107 3076 1382
Vertebrata 789 254 535 427
Mammals 238 33 205 124

Number of Variants Analyzed:

Protein Length Pathogenic Variants Benign/Unknown Variants
739 107 648

Domains

Domains
Current Study 1, 110
Guo et al (2010) 2, 61

Results

Mutations in Ancient vs Modern Domains

Current Study

Ancient Domains Modern Domains All Ratio
Number of Residues 630 109 739 5.779817
Number of Variants 601 154 755 3.902597
Number of Pathogenic Variants 96 11 107 8.727273
Number of Benign/Unknown Variants 505 143 648 3.531469
Fisher exact test 0.004182

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Guo et al (2010)

Ancient Domains Modern Domains All Ratio
Number of Residues 679 60 739 11.316667
Number of Variants 658 97 755 6.783505
Number of Pathogenic Variants 102 5 107 20.400000
Number of Benign/Unknown Variants 556 92 648 6.043478
Fisher exact test 0.004603

The Fisher exact test was calculated using a contingency table with the number of pathogenic and benign/unknown variants in ancient and modern domains. The bottom left values.

Coverage of the alignment and domain locations

Agreement between the current study and Guo et al (2010)

Conservation of the amino acids in modern and ancient domains

# of sites Ancient # sites Modern Mean Ancient (SD) Mean Modern (SD) Median Ancient Median Modern Mann-Whitney U p-value
Current Study 630 109 0.95 (0.00) 0.73 (0.02) 9.879e-01 7.370e-01 58903.00 1.83e-33
Guo et al (2010) 679 60 0.94 (0.00) 0.60 (0.02) 9.870e-01 5.724e-01 39163.00 4.96e-33

The Mann-Whitney U test was calculated using the conservation scores of the amino acids in ancient and modern domains. The p-value is for the null hypothesis that the conservation scores in ancient domains are greater than in modern domains.

Conservation of the amino acids in modern and ancient domains